The experimentation clearly revealed that both CHM-Gn media donâ€™t absolutely fulfill prospects considering VRE exposure, particularly when inoculating stool samples straight or following pre-dilution. Irresistible cultivation of diverse bacteria organisms and yeasts on media â€œAâ€ and â€œBâ€ hinder agar assessment while culturing stools samples directly. Stool samples are therefore unsuitable for straight inoculation onto both media. â€œOvernight stool pre-inoculation to facilitate enrichment is paramount prior to plating samples onto both media (3). Projected major reactions support the key classification of doubtful isolates, hence, therefore, greatly recommended in shunning pseudo positive outcomes and verification of vancomycin resistance.
Analysis of colony color â€œBâ€ revealed colony color stability during the 24 and 48 hrs growth periods. This is however unexpected according to CHM-Gn â€œAâ€ manufacturers. On â€œAâ€ medium, the appearance of different color shades of VRE species further complicates the analysis. Regrettably, instituting the phase of enrichment slowed VRE identification. Fortunately, using either media presents a VRE cost-cutting screening technique for stool analysis, hence safety measures decreasing detrimental infection prevalence.
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