The research under analysis is aimed at studying the sake yeast strainsâ€™ mitochondrial activities as well as their metabolic engineering that is likely to have an impact on their capacity to ferment substrates. Thus, the principal purpose of the relevant study resides in illustrating the positive interconnection that exists between the inhibited mitophagy and the fermentative capacity. Moreover, the researchers intend to receive some findings that can be potentially transformed into valuable recommendations for the relevant industry.
According to the authors, their approach is of innovative character and can be used for increasing the brewery yeastsâ€™ fermentative capacity (Shiroma et al. 1003).
The researchers employ a variety of methods in order to reach the targeted objectives. Thus, one of the principal methods employed in the study is the observation that enables them to monitor the process and its progress. Further on, a series of measurements are carried out to assess the weight of dry cells, cellsâ€™ area, and the mitochondriaâ€™s signal intensity. After performing a quantitative reverse transcriptase real-time PCR, the researchers use statistical analysis to generalize the obtained findings. In the framework of the relevant analysis, the two-sample z-test is employed in order to evaluate the statistical relevance of the difference between the sample groups.
The carried out study lets the researchers receive a series of results. Thus, first of all, they learn that mitophagyâ€™s occurrence depends considerably on Atg32. According to the researchers, the relevant mitophagy is essential for maintaining the quantity of mitochondrial in sake yeast in the course of its brewing (Shiroma et al. 1008). Moreover, the first finding lets them discover another phenomenon â€“ mitophagy plays a considerable role in process of the fermentation of ethanol. In other words, mitophagy proves to have an impact not only on the fermentation process but its outcomes as well.
In the course of the research, it is also found out that laboratory and sake yeasts tend to respond in a different manner the concentrations of nutrient. Therefore, the targeted balance might be reached with the regulation of the ethanol concentration.
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