The test item was >98% pure as stated by the sponsor but no certificate of analysis was received. The sponsor also asked that the higher dose of the test item be administered at 600mg/ kg/ day instead of 700mg/ kg/day. Due to experimental errors during the study, rats from group 1(vehicle) were administered vehicle twice instead of once on the 14thÂ day of dosing. In period of acclimatization, some males and females were housed together for a day and fortunately, assessment of the females during the study indicated that they were not pregnant and hence did not affect the outcomes of the study. Three rats that received low dose of the test item were not given any water for 24 hours before necropsy. The humidity of the room the rats were housed reached 90% on the 14thÂ day of dosing.
This is where there is no direct link between the sender and the receiver of the messages. It is also called non-personal form of communication and it includes the use of events, the atmosphere and media. In this case, atmosphere refers to what a firm creates in their different offices. The office exteriors and interiors have a great meaning to potential customers.
Media channels here are the broadcast media (television, radio), the print media (souvenirs, magazines, proceedings of conferences, newspapers), electronic media and display media. Events can be defined as occurrences planned to communicate specific messages to target audiences. For example, a company may set opening ceremonies of different kinds, sponsorships of different events and news conferences.
Experimental errors during the study should be avoided by ensuring that everything taking place during the study and in the lab is recorded. Before undertaking any activity, all records should be reviewed to avoid doing mistakes like the double administration of the vehicle or test substance. According to Tupper and Wallace (1980), males and females should also never be housed together. Further more, cage temperatures, light and humidity should be strictly controlled at the recommended level (Gad, 1991).
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